Infinite. Total Magnification: 100-1000X. 10X High Eyepoint Eyepiece. 10X 20X 40X 100X Infinity Plan Achromatic Phase Contrast Objective. Standard Coupler: 0.5X. Eye Tube Angle: 30°. Eyepiece Field of View: Dia. 22mm. XY Stage Travel Distance: 78x54mm. Illumination Type: LED Coaxial Transmitted Light. Monitor Display Function: Operation/Lock/Auto Sleep/Standby. Input Voltage: AC 100-240V 50/60Hz.
|Phase contrast microscope is a kind of microscope that observes the object using a condenser with an annular diaphragm and a phase difference objective with a phase plate by increasing the contrast of the image by changing the optical path that the diffracted light passes. |
The principle of phase contrast microscope is that, under the illumination of the same or similar intensity of light, tiny or microscopic objects (such as living cells and unstained biological specimens) cannot distinguish their difference characteristics, therefore through the small height difference (about 100-1500 angstroms) of their surface, by using the diffraction and interference characteristics of light, increase the contrast of microscope imaging by adding phase contrast ring plates and other accessory devices.
After being focused by the condenser, the illumination light is projected on an annular diaphragm, becoming a tubular beam. By using the height difference of the surface of the object to be observed, the direct light and the diffracted light are separated, and after it is overlapped with the phase plate through the auxiliary lens, about 1/2 of the wavelength is removed from the phase, which makes them impossible to interact, thereby causing changes in intensity, improving the contrast of the specimen observation, and making the various structures clearer.
The difference between phase contrast microscope and ordinary biological or metallurgical microscope is that the variable diaphragm is replaced by annular diaphragm, and the ordinary objective lens is replaced by the objective lens with the phase plate, providing a phase telescope for coupling axis as the main accessory of the phase contrast microscope.
The illumination of a phase contrast microscope requires, first of all, a stronger source of light because the annular diaphragm and the phase plate block and absorb most of the light, and the light entering the imaging diaphragm is relatively weak. Secondly, it is necessary to use Kohler illumination to evenly focus the light on the aperture diaphragm, and ensure the temperature environment of the observed living cells, etc., and further eliminate the heat radiation by adding a heat insulating filter.
In addition, when observing biological specimens, it is best to use a color filter (usually a green color filter). Use a monochromatic light source to ensure adjustment of the refractive index difference of the biological specimen. The image effect produced by the phase contrast microscope is that the bright and dark structural features of the samples can be displayed on a gray background.
Phase contrast microscopes can be divided into transmissive phase contrast microscopes (such as phase contrast biological microscopes) and reflective phase contrast microscopes (such as phase contrast metallurgical microscopes).
By increasing the contrast of the microscope, transmitted light phase contrast microscope can observe living cells and biological samples without staining. It can also be used to observe stained samples with small contrast and small organ tissues. Transmitted light phase contrast microscope is widely used in the fields of biology and pathology such as cells and bacteria.
Reflected light phase contrast microscope can observe the microstructure of microscopic height difference which is not easy to be distinguished by ordinary metallurgical microscope, such as metallurgic, crystal, oil film, chemicals, dust particles, and materials science and other aspects.
Precautions in the Use of Phase Contrast Microscope
1. The thickness of the specimen should be less than 5μm. When the specimen is relatively thick, the upper layer is observed to be clear, but the deep layer will be blurred, and cause phase displacement interference and light scattering interference.
2. For the specimen, cover slip should be used, otherwise the bright ring of the annular diaphragm and the dark ring of the phase plate will be difficult to overlap due to the change of the optical path of the objective lens. At the same time, there is also relatively higher requirements for the quality of slides and coverslips. When surface scratches or irregularities occur, it will produce bright ring skew and phase interference. When the slide is too thick or too thin, it will also affect the size of the ring diaphragm of the annular diaphragm.
For more precautions for use of phase contrast microscope, please refer to the Biological Microscope on the BoliOptics website.
|Microscopes and components have two types of optical path design structures.|
One type is finite optical structural design, in which light passing through the objective lens is directed at the intermediate image plane (located in the front focal plane of the eyepiece) and converges at that point. The finite structure is an integrated design, with a compact structure, and it is a kind of economical microscope.
Another type is infinite optical structural design, in which the light between the tube lens after passing the objective lens becomes "parallel light". Within this distance, various kinds of optical components necessary such as beam splitters or optical filters call be added, and at the same time, this kind of design has better imaging results. As the design is modular, it is also called modular microscope. The modular structure facilitates the addition of different imaging and lighting accessories in the middle of the system as required.
The main components of infinite and finite, especially objective lens, are usually not interchangeable for use, and even if they can be imaged, the image quality will also have some defects.
The separative two-objective lens structure of the dual-light path of stereo microscope (SZ/FS microscope) is also known as Greenough.
Parallel optical microscope uses a parallel structure (PZ microscope), which is different from the separative two-object lens structure, and because its objective lens is one and the same, it is therefore also known as the CMO common main objective.
|The tube lens focal length is the focal length from the tube lens to the intermediate image plane of the design of infinite microscope, and its typical ranging is from 160 to 200 mm, depending on different manufacturers.|
|The magnification of the objective lens refers to the lateral magnification, it is the ratio of the image to the real size after the original image is magnified by the instrument. This multiple refers to the length or width of the magnified object.|
System optical magnification is the product of the eyepiece and the objective lens (objective lens zoom set) of the optical imaging part within the system.
Optical magnification = eyepiece multiple X objective lens/objective lens set
The maximum optical magnification of the microscope depends on the wavelength of the light to which the object is illuminated. The size of the object that can be observed must be greater than the wavelength of the light. Otherwise, the light cannot be reflected or transmitted, or recognized by the human eye. The shortest wavelength of ultraviolet light is 0.2 microns, so the resolution of the optical microscope in the visible range does not exceed 0.2 microns, or 200 nanometers. This size is converted to the magnification of the microscope, and it is the optical magnification of 2000X. Usually, the compound microscope can achieve 100X objective lens, the eyepiece is 20X, and the magnification can reach 2000X. If it is bigger, it will be called "invalid magnification", that is, the image is large, but the resolution is no longer increased, and no more details and information can be seen.
|When the instrument is conducting electronic image magnification and observation through a camera or the like, the optically magnified portion may not be the optical path that passes through the "eyepiece-objective lens" of the instrument, at this time, the calculation method of the magnification is related to the third-party photo eyepiece passed. |
The trinocular optical magnification is equal to the multiplier product of objective lens (objective lens set) and the photo eyepiece
Trinocular optical magnification = objective lens X photo eyepiece
|Total magnification is the magnification of the observed object finally obtained by the instrument. This magnification is often the product of the optical magnification and the electronic magnification.|
When it is only optically magnified, the total magnification will be the optical magnification.
Total magnification = optical magnification X electronic magnification
Total magnification = (objective X photo eyepiece) X (display size / camera sensor target )
|Field of View, is also called FOV. |
The field of view, or FOV, refers to the size of the object plane (i.e., the plane of the point of the observed object perpendicular to the optical axis), or of its conjugate plane (i.e., object to primary image distance), represented by a line value.
System field of view is the size of the actual diameter of the image of the terminal display device of the instrument, such as the size of the image in the eyepiece or in the display.
Field of view number refers to the diameter of the field diaphragm of the objective lens, or the diameter of the image plane formed by the field diaphragm.
Field of view number of objective lens = field of view number of eyepiece / (objective magnification / mechanical tube length)
Large field of view makes it easy to observe the full view and more range of the observed object, but the field of view (FOV) is inversely proportional to the magnification and inversely proportional to the resolution, that is, the larger the field of view, the smaller the magnification, and also the lower the resolution of the object to be observed.
There are usually two ways to increase the field of view, one is to replace with an objective lens of a smaller multiple, or to replace with an eyepiece of a smaller multiple.
|Working distance, also referred to as WD, is usually the vertical distance from the foremost surface end of the objective lens of the microscope to the surface of the observed object.|
When the working distance or WD is large, the space between the objective lens and the object to be observed is also large, which can facilitate operation and the use of corresponding lighting conditions.
In general, system working distance is the working distance of the objective lens. When some other equipment, such as a light source etc., is used below the objective lens, the working distance (i.e., space) will become smaller.
Working distance or WD is related to the design of the working distance of the objective lens. Generally speaking, the bigger the magnification of the objective lens, the smaller the working distance. Conversely, the smaller the magnification of the objective lens, the greater the working distance.
When it is necessary to change the working distance requirement, it can be realized by changing the magnification of the objective lens.
|For siedentopf eyetube, when changing the interpupillary distance, it requires two hands pushing or pulling the two eyetubes left and right simultaneously, and the two eyepiece tubes or eyetubes will change their position at the same time.|
|Usually the Microscope Eyetube is 45°, some is 30°, Tiltable Eyetube Angle design of a microscope is also known as the ergonomics microscope.|
0-30° or 0-45° is an ergonomic design. When the mechanical tube length / focal length of the tube of the microscope is relatively big, the microscope is relatively high, and the user's height or the seat of the work desk is not suitable, long-term use of microscope may cause sitting discomfort.
Eyepiece tube with variable angle can freely adjust the angle without lowering the head. Especially when it is close to 0 degree and the human eye is close to horizontal viewing, long-time or long-term use can avoid fatigue damage to the cervical vertebra.
|After imaging through a set of objective lenses, the object observed and the image seen by the human eye is inverted. When the observed object is manipulated, move the specimen or object, the image will move in the opposite direction in the field of view. Most of the biological microscopes are reversed-phase designs.|
When needing to operate works with accurate direction, it is necessary to design it into a forward microscope. Generally stereo microscopes and metallurgical microscopes are all of erect image design.
When observing through the camera and display, the erect and inverted image can be changed by the orientation of the camera.
|The eyepiece of the microscope can have different viewing or observing directions. When the position of the microscope is uncomfortable, the direction of the eyepiece tube of the microscope can be adjusted, to facilitate observation and operation.|
Placement method of different viewing angles of the microscope:
General direction: the support column is behind the object to be observed
Reverse direction: the support column is in front of the object to be observed
Lateral direction: the support column is on the side of the object to be observed
Rotating eyepiece tube, different microscopes may have different methods, for some, the direction is confirmed when installing the eyepiece tube of the microscope, for some, by rotating the body of the microscope, and for some, by rotating the support member on the support or holder of the microscope.
|The distance between the two pupils of the human eye is different. When the image of exit pupil of the two eyepieces of the microscope are not aligned with the entry pupil of the eye, the two eyes will see different images, which can cause discomfort.|
Adjust the distance between the two eyepieces, to accommodate or adapt to the pupil distance of the observer's eyes. The adjustment range is generally between 55-75mm.
|The third eyepiece splitting in the trinocular microscope is to borrow one of the two sets of eyepiece optical paths as the photographic light path. The beam split prism or beam splitter can reflect part of the image light to the eyepiece, and part passes through to the third eyepiece photographic light path, such a trinocular microscope is called trinocular simultaneous imaging microscope, or true-trinocular.|
The beam split prism or beam splitter of the trinocular simultaneous imaging microscope or true-trinocular often has different splitting modes, such as 20/80 and 50/50, etc. Usually, the former is the luminous flux ratio of the eyepiece optical path, and the latter is the luminous flux ratio of the photographic optical path.
The advantage of true-trinocular is that, the real three optical paths can be imaged at the same time, and are not affected by the simultaneous use of the eyepiece observation and the photographic optical path (display). The disadvantage is that, because of the reason of the splitting, the image light of the photography is only a part. In theory, the image effect will be affected, and the effect is more obvious in the binocular eyepiece observation. If viewed closely, one will find that the eyepiece of the light path is relatively dark. However, in the current optical design and materials, the impact on the actual work is not very big, especially in the observation of low magnification objective lens, it has basically no effect at all, and therefore used by many people.
|The adjustable eyepiece is between the lens of the eyepiece and the focal plane, with distance adjustable device.|
For most people, their two eyes, the left and the right, have different vision. For adjustable eyepieces, the eyepoint height of the eyepiece can be adjusted to compensate for the difference in vision between the two eyes, making the image in the two eyes clear and consistent.
The range of adjustment of the general eyepiece is that the diopter is plus or minus 5 degrees, and the maximum difference between the two eyepieces can reach 10 degrees. Before use, it is generally necessary to adjust both eyepieces to the initial position where the scale is displayed as 0, which is used as a baseline to facilitate up and down adjustment.
The reticle position of the eyepiece is generally 10mm below the fixed position of the eyepiece tube. Because the vision of each person is different, some people may not be able to see the reticle clearly. For adjustable eyepiece, the height of the reticle position can be adjusted to make the reticle and the observed object clear at the same time, this is the advantage of adjustable eyepiece that mounts the diopter adjustment on the eyepiece tube compared with non-adjustable eyepiece.
When non-adjustable eyepiece is equipped with a reticle, if the diopter is adjusted, the reticle will rotate accordingly, thereby affecting the position of the measurement. For adjustable eyepiece, when its diopter is adjusted, its reticle does not rotate.
|Eyepiece optical magnification is the visual magnification of the virtual image after initial imaging through the eyepiece. When the human eye observes through the eyepiece, the ratio of the tangent of the angle of view of the image and the tangent of the angle of view of the human eye when viewing or observing the object directly at the reference viewing distance is usually calculated according to 250 mm/focal length of eyepiece.|
The standard configuration of a general microscope is a 10X eyepiece.
Usually, the magnification of the eyepiece of compound microscope is 5X, 8X, 10X, 12.5X, 16X, 20X.
As stereo microscope has a low total magnification, its eyepiece magnification generally does not use 5X, but can achieve 25X, 30X and other much bigger magnification.
|The eyepiece field of view is the diameter of the field diaphragm of the eyepiece, or the diameter of the image plane of the field diaphragm imaged by the field diaphragm.|
The diameter of a large field of view can increase the viewing range, and see more detail in the field of view. However, if the field of view is too large, the spherical aberration and distortion around the eyepiece will increase, and the stray light around the field of view will affect the imaging effect.
|Eye point refers to the axial distance between the upper end of the metal frame of the eyepiece and the exit of pupil.|
The exit of pupil distance of high eyepoint eyepiece is farther than that of the eye lens of the ordinary eyepiece. When this distance is greater than or equal to 18mm, it is a high eyepoint eyepiece. When observing, one does not need to be too close to the eyepiece lens, making it comfort to observe, and it can also be viewed with glasses. Generally, there is a glasses logo on the eyepiece, indicating that it is a high eyepoint eyepiece.
|The finite objective is the lateral magnification of the primary image formed by the objective at a prescribed distance.|
Infinite objective is the lateral magnification of the real image produced by the combination of the objective and the tube lens.
Infinite objective magnification = tube lens focal length (mm) / objective focal length (mm)
Lateral magnification of the image, that is, the ratio of the size of the image to the size of the object.
The larger the magnification of the objective, the higher the resolution, the smaller the corresponding field of view, and the shorter the working distance.
|In the case of polychromatic light imaging, the aberration caused by the light of different wavelengths becomes chromatic aberration. Achromatic aberration is to correct the axial chromatic aberration to the two line spectra (C line, F line); apochromatic aberration is to correct the three line spectra (C line, D line, F line).|
The objective is designed according to the achromaticity and the flatness of the field of view. It can be divided into the following categories.
Achromatic objective: achromatic objective has corrected the chromatic aberration, spherical aberration, and comatic aberration. The chromatic portion of the achromatic objective has corrected only red and green, so when using achromatic objective, yellow-green filters are often used to reduce aberrations. The aberration of the achromatic objective in the center of the field of view is basically corrected, and as its structure is simple, the cost is low, it is commonly used in a microscope.
Semi-plan achromatic objective: in addition to meeting the requirements of achromatic objective, the curvature of field and astigmatism of the objective should also be properly corrected.
Plan achromatic objective: in addition to meeting the requirements of achromatic objectives, the curvature of field and astigmatism of the objective should also be well corrected. The plan objective provides a very good correction of the image plane curvature in the field of view of the objective, making the entire field of view smooth and easy to observe, especially in measurement it has achieved a more accurate effect.
Plan semi-apochromatic objective: in addition to meeting the requirements of plan achromatic objective, it is necessary to well correct the secondary spectrum of the objective (the axial chromatic aberration of the C line and the F line).
Plan apochromatic objective: in addition to meeting the requirements of plan achromatic objective, it is necessary to very well correct the tertiary spectrum of the objective (the axial chromatic aberration of the C line, the D line and the F line) and spherochromatic aberration. The apochromatic aberration has corrected the chromatic aberration in the range of red, green and purple (basically the entire visible light), and there is basically no limitation on the imaging effect of the light source. Generally, the apochromatic aberration is used in a high magnification objective.
|Objective parfocal distance refers to the imaging distance between the objective shoulder and the uncovered object surface (referred to as the “object distance). It conforms to the microscope design, usually 45mm. |
The objective of different magnifications of the compound microscope has different lengths; when the distance between the objective shoulder and the object distance is the same, the focal length may not be adjusted when converting to objectives of different magnifications.
|The objective working distance is the vertical distance from the foremost surface end of the objective of the microscope to the object surface to be observed.|
Generally, the greater the magnification, the higher the resolution of the objective, and the smaller the working distance, the smaller the field of view. Conversely, the smaller the magnification, the lower the resolution of the objective, and the greater the working distance, and greater the field of view.
High-magnification objectives (such as 80X and 100X objectives) have a very short working distance. Be very careful when focusing for observation. Generally, it is after the objective is in position, the axial limit protection is locked, then the objective is moved away from the direction of the observed object.
The relatively greater working distance leaves a relatively large space between the objective and the object to be observed. It is suitable for under microscope operation, and it is also easier to use more illumination methods. The defect is that it may reduce the numerical aperture of the objective, thereby reducing the resolution.
|Numerical aperture, N.A. for short, is the product of the sinusoidal function value of the opening or solid angle of the beam reflected or refracted from the object into the mouth of the objective and the refractive index of the medium between the front lens of the objective and the object.|
Simply speaking, it is the magnitude of the luminous flux that can be brought in to the mouth of the objective adapter, the closer the objective to the specimen for observation, the greater the solid angle of the beam entering the mouth of the objective adapter, the greater the N.A. value, and the higher the resolution of the objective.
When the mouth of the objective adapter is unchanged and the working distance between the objective and the specimen is constant, the refractive index of the medium will be of certain meaning. For example, the refractive index of air is 1, water is 1.33, and cedar oil is 1.515, therefore, when using an aqueous medium or cedar oil, a greater N.A. value can be obtained, thereby improving the resolution of the objective.
N.A. = refractive index of the medium X sin solid angle of the beam of the object entering the front lens frame of the objective/ 2
Numerical aperture of the objective. Usually, there is a calculation method for the magnification of the microscope. That is, the magnification of the microscope cannot exceed 1000X of the objective. For example, the numerical aperture of a 100X objective is 1.25, when using a 10X eyepiece, the total magnification is 1000X, far below 1.25 X 1000 = 1250X, then the image seen in the eyepiece is relatively clear; if a 20X eyepiece is used, the total magnification will reach 2000X, much higher than 1250X, then eventhoughthe image actually seen by the 20X eyepiece is relatively large, the effect will be relatively poor.
|The thickness of the cover glass affects the parfocal distance of the objective. Usually, in the design of the focal length of the objective,the thickness of the cover glass should be considered, and the standard is 0.17mm.|
|The use of different media between the objective and the object to be observed is to change and improve the resolution. For example, the refractive index of air is 1, water is 1.33, and cedar oil is 1.515. Therefore, when using an aqueous medium or cedar oil, a greater N.A. value can be obtained, thereby increasing the resolution of the objective.|
Air medium is called dry objective, where oil is used as medium iscalled oil immersion objective, and water medium is called water immersion objective.
However, because of the working distance of the objective, when the working distance of the objective is too long, the use of liquid medium will be relatively more difficult, and it is generally used only on high magnification objective having a shorter working distance, such as objectives of 60X, 80X and 100X.
When using oil immersion objective, first add a drop of cedar oil (objective oil) on the cover glass, then adjust the focus (fine adjustment) knob, and carefully observe it from under the side of the objective of the microscope, until the oil immersion objective is immersed in the cedar oil and close to the cover glass of the specimen, then use the eyepiece to observe, and use the fine focus knob to lift the tube until the clear imageof the specimen is clearly seen.
The cedar oil should be added in an appropriate amount. After the oil immersion objective is used, it is necessary to use a piece of lens wiping tissue to dip xylene to wipe off the cedar oil, and then wipe dry the lens thoroughly with a lens wiping tissue.
|The front end of the objective is equipped with a spring device. When the working distance of the objective is too short, focusing can easily make the objective contact the object to be observed, thereby damaging the object to be observed or the front lens. At this time, the spring acts to recover the front end of the objective lens. It is usually used on high magnification objectives with very short working distances.|
|For microscopes of different manufacturers and different models, the thread size of their objectives may also be different. |
In general, the objective threads are available in two standard sizes, allowing similar objectives between different manufacturers to be used interchangeably.
One is the British system: RMS type objective thread: 4/5in X 1/36in,
One is metric: M25 X 0.75mm thread.
|The nosepiece has a slot for mounting polarizers, filters and other devices.|
|Illumination base is a modular light source component, suitable for microscope stand base that has no light source of itself, and it is usually dedicated components supporting some stands.|
Illumination base typically includes at least one bottom lighting, and there are also illumination base that includes the circuit portion of the upper light source.
|Focus mechanism, the coarse / fine focus knobs are in a coaxial center position, they are connected together by a gear reduction mechanism, which can be coarse/ fine focus adjusted at any time during the entire stroke.|
Generally, the coarse focus diameter is relatively big, which is inside close to the body of the microscope, and the fine focus diameter is relatively small, which is outside of the body of the microscope. Coarse focus adjustment is used to quickly move to find the image, and the fine focus adjustment is used to finely adjust the clarity of the image. Generally, the minimum read value of the fine focus adjustment can be accurate to 1 micron, and single circle can reach a stroke of 0.1 mm. Mechanical fine focus plays a very important role in the accuracy of the microscope resolution. If the fine focus accuracy is not enough, or cannot be stabilized at the sharpest focusing position, the image will be out of focus and become blurred.
The tightness of coarse focus is generally adjustable. Generally, on one side of the knob (usually on the right side), there is a textured knob on the inside of the coarse knob, which is tightened if rotated clockwise; and loosened if rotated counterclockwise.
In the process of focusing, direct focusing should not be on the objective of high magnification; instead, find the object of low magnification first, and gradually adjust to high magnification. Usually, the coarse focus knob is rotated first, and when the objective lens is gradually lowered or the platform is gradually rising, find the object, and then adjust with the fine focus, until the object image in the field of view is clear. Generally, when changing from low magnification to high magnification objective, one only need to slightly adjust the fine focus knob to make the object image clear. During the process, the distance between the objective and the specimen should be observed from the side, to understand the critical value of the object distance between the lens and the specimen.
When using a high magnification objective, since the distance between the objective and the specimen is very close, after the image is found, the coarse focus knob cannot generally be used, and the fine focus knob can only be used to avoid excessive distance of movement, damaging the objective and the slide or specimen.
By using the characteristics of the fine focus, the height or thickness of the observed object can be roughly measured under the microscope, such as measuring the thickness of the cell or tissue, the thickness of the cover glass, and the thickness of small objects that cannot be measured by various conventional measuring instruments.
Method of measurement: place the object to be measured at the center of the field of view of the stage. After the image is clearly focused, try to use the highest magnification objective as much as possible, and align the adapter of the top feature point of the object to be measured. After adjusting clear, record the position of scale of the fine focus knob. Then, move the objective down to the adapter of the lowest feature point of the object to be measured, and record the position of scale of the fine focus knob. Then, according to the above fine focus, record the number of rounds of movement, and based on the parameters of conversion of each round into stroke (see the microscope fine focus knob parameters), the number of rounds is converted into the total stroke, which is the height of the object to be measured. If it is repeated a few times for average, a more accurate measurement can be obtained.
|Mostly, at the junction of the compound microscope platform and the body, there is a longitudinal limit mechanism. When the limit mechanism is locked, the platform is prevented from moving up and colliding with the microscope objective, thereby damaging the specimen or destroying the lens.|
On its first use, use one specimen, applying 100X or the highest magnification lens, carefully find the clearest image, then lock the axial limit mechanism down, the focus mechanism will remember this position. When the focus is adjusted again to reach this position in the future, it will not go up again, and the platform or specimen will not touch the lens.
|Different microscope bodies, different human operations, and different requirements for observation and operation, all require adjustment of the pre-tightening force of the stand that support microscope body.|
Facing the stand just right, use both hands to reverse the force to adjust the tightness. (face the knob of one side just right, clockwise is to tighten, counterclockwise is to loosen)
In general, after long-time use, the knob will be loose, and adjustment is necessary.
|Stage backlight window size refers to the size of the window through which the transmitted light passes under the stage on the XY table plane of the stage.|
This window is usually covered with a piece of glass. For some stages with accuracy requirements in the XY horizontal direction, the horizontal plane of the glass can be adjusted by the height of the screws on the four corners below, and the consistency with the height of the stage plane is guaranteed.
|The movement of the microscope stage or the mechanical stage can be measured by the moving distance of the ruler, and the size and area of the sample details can be calculated.|
The ruler can be divided into main scale and sub-scale. The minimum grid value of the main scale is 1 mm, the integer is measured; the minimum grid value of the sub-scale is 0.9 mm, the decimal is measured. When measuring, if what the main ruler measures is not an integer and therefore one needs to read the decimal of the specimen, align the end point of the sub-scale to the end of this specimen, and then find the scale on the line of main ruler and the sub-ruler, and see which group is the closest, the length of this decimal is the reading of the sub-scale.
|Kohler illumination: is a secondary imaging illumination that overcomes the shortcoming of direct illumination of critical illumination. After the filament of the light source passes through the condenser and the variable field diaphragm, the filament image falls for the first time in the condenser aperture diaphragm, the condenser forms a second image at the back focus plane position there, so that there is no filament image at the plane of the object to be observed, and the illumination becomes uniform.|
During observation, by changing the size of the condenser aperture diaphragm, the light source fills in the entrance pupil of the objective lens, and the numerical aperture of the condenser is matched with the numerical aperture of the objective lens. At the same time, the condenser images the field diaphragm at the plane of the observed object, and the illumination range is controlled by the size of the field diaphragm. Since the thermal focus of Kohler illumination is not at the plane of the object to be observed, the object to be observed will not be damaged even if it is irradiated for a long time.
|The diaphragm that determines the image plane necessary for imaging through the objective lens is called the aperture diaphragm. All irises of the traditional microscope are aperture diaphragm.|
The function of aperture diaphragm is mainly to limit the size of the imaging beam, change the luminous flux, thereby improving the imaging quality. The size of the aperture diaphragm is usually variable, and it is also called iris diaphragm. When the aperture diaphragm lock is too small and the luminous flux of the imaging beam is insufficient, the fraction ratio of the objective lens is low, the imaging will become dark; however, when the aperture diaphragm is too large, there will be strong light in the field of view, and even though viewed from the eyepiece, it may have high resolution, the image on the display will be overexposed.
After replacing the objective lens, the aperture diaphragm should also be adjusted appropriately, rather than adjusting the brightness of the light.
The aperture diaphragm of the transmitted light is generally mounted on the microscope base. The aperture diaphragm of the biological microscope is mounted on the condenser device. On the other hand, the aperture diaphragm of compound microscopes, such as large upright metallurgical or fluorescence microscopes, is generally mounted on the in the coaxial reflection illuminator.
In the use of the aperture diaphragm, it is often necessary to adjust the center of the diaphragm. Generally, it is adjusted together with the condenser. Please refer to the adjustment method of the condenser.
|Field diaphragm is also called field of view diaphragm, field of view cutting diaphragm. |
The diaphragm that defines the incident angle of view and the exit angle of view of the beam emitted from the object plane, is called field diaphragm.
The main function of the field diaphragm is to limit the range of the image surface size of the observed specimen, and cut off the part of the image edge image plane with relatively poor image quality, so that the entire image plane is clear and flat, but does not affect the resolution of the entire objective lens.
The appropriate adjustment of the field diaphragm can also adjust the glare reflected from the inner wall of the lens tube to improve the imaging contrast and quality. On the eyepiece of the microscope, there is a field-cutting diaphragm. The size of this diaphragm is fixed, and it is also called fixed diaphragm. Its position is between the field lens and the eyepiece, and its function is to limit the emit angle of view of the main beam, so as to make the imaging of the field edge to achieve an ideal effect.
The field diaphragm of most biological microscopes is on the light exit of the base, while the field diaphragm of compound microscopes, such as upright metallurgical and fluorescent microscope, are mounted on the coaxial reflection illuminator.
|Phase contrast kit mainly includes annular diaphragm, phase contrast objective with a phase plate, and a phase telescope for central alignment.|
The annular diaphragm is placed near the aperture diaphragm, and there are single pieces, some are combined into a diaphragm group. There are also some that make the annular diaphragm into a turntable and combine together with the condenser. The annular diaphragm is to use a glass, coated with metal film, to block the light. When the light passes through the narrow slit of the diaphragm, it forms a hollow light cone, and different diaphragms are used corresponding to different objective lenses so as to generate diffraction and interference effects.
The phase plate is mounted on the back image focal point of the objective lens. It is to use a piece of glass, coated with a light absorbing material (magnesium fluoride or other electrolyte) and a layer of metal film at the ring that is transparent to the corresponding annular diaphragm, so that the phase of direct or diffracted light passing through the phased annular diaphragm can be delayed by 1/4λ. The phase plate absorbs part of the light, extends the optical path and delays the phase of part of the light. After the two beams of light are combined, the interference is strengthened, and the vibration amplitude is increased or decreased, resulting in a phase difference, which makes various structural features more clear.
Phase plates are usually used in two forms:
Negative contrast: delay the direct light by 1/4λ. After the two sets of light waves are combined, the light waves are added, the vibration amplitude is increased, and the specimen structure becomes much brighter than the surrounding medium, forming a bright contrast.
Positive contrast: delay the diffracted light by 1/4λ. After the two sets of light are combined, the light waves are subtracted, the vibration amplitude is reduced, and the specimen structure becomes much darker than the surrounding medium, forming a dark contrast.
Annular diaphragm and phase plate adjustment
Phase telescope works on the coaxial correction of the annular diaphragm and the phase plate. When using a phase-aligned telescope, it temporarily replaces one eyepiece, adjust and focus the image of the object on the phase plate, and then observe and adjust the auxiliary lens of the condenser. Adjust the annular diaphragm and the right corresponding to the phase plate to its concentric position, so that the beam of the annular diaphragm and the phase ring of the phase plate are the same size, and the annular beam is completely projected on the phase ring.
Some microscopes have a Berrand lens that can be switched in the optical path, which is to correct the position of the annular diaphragm and phase plate. When using, screw the Bertrand lens into the optical path, adjust the focus and see the images of the annular diaphragm and the phase plate, after adjusting its position, rotate the Bertrand lens out of the optical path.
|Abbe condenser is a kind of bright field condenser, a condenser that can only finitely correct the spherical aberration, but not the chromatic aberration. When the numerical aperture of its objectives is higher than 0.6, Abbe condenser will show chromatic aberration and spherical aberration.|
|Coupler/C-mount adapter is an adapter commonly used for connection between the C-adapter camera (industrial camera) and a microscope.|
|On the coupler/C-mount-adapter, there is an adjustable device to adjust the focal length.|
|Different coupler/C-mount-adapters are suitable for different microscopes. For some, some adapter accessories need to be replaced. See the applicable range of each coupler/C-mount-adapter for details.|
|Coupler magnification refers to the line field magnification of the coupler/C-mount-adapter. With different magnifications of the adapter lens, images of different magnifications and fields of view can be obtained. The size of the image field of view is related to the sensor size and the coupler/C-mount-adapter magnification.|
Camera image field of view (mm) = sensor diagonal / coupler/C-mount-adapter magnification.
For example: 1/2 inch sensor size, 0.5X coupler/C-mount-adapter coupler, field of view FOV (mm) = 8mm / 0.5 = 16mm.
The field of view number of the microscope 10X eyepiece is usually designed to be 18, 20, 22, 23mm, less than 1 inch (25.4mm). Since most commonly used camera sensor sizes are 1/3 and 1/2 inches, this makes the image field of view on the display always smaller than the field of view of the eyepiece for observation, and the visual perception becomes inconsistent when simultaneously viewed on both the eyepiece and the display. If it is changed to a 0.5X coupler/C-mount-adapter, the microscope image magnification is reduced by 1/2 and the field of view is doubled, then the image captured by the camera will be close to the range observed in the eyepiece.
Some adapters are designed without a lens, and their optical magnification is considered 1X.
|For the size of the lens field of view of the coupler/C-mount-adapter, in the design process, the size of the camera sensor imaging target should be considered. When the field of view of the lens is smaller than the target plane of the camera, “black border” and “dark corner” will appear.|
The general microscope coupler/C-mount adapters are generally designed for the 1/2" camera targets. When a camera of 2/3 or larger target is used, the “dark corner” phenomenon will appear in the field of view. Especially, at present, DSLR cameras generally use large target plane design (1 inch full field of view), when used for microscopic photographing, the general DSLR camera coupler/C-mount adapter will have “black border”.
Generally, the “dark corner” that appears on the field of view is often that the center of the microscope and the camera are not aligned. Adjust the position of the screw on the camera adapter, or turn the camera adapter to adjust or change the effect.
|At present, the coupler/C-mount adapter generally adopts the C/CS-Mount adapter to match with the industrial camera. For details, please refer to "Camera Lens Mount".|
|After unpacking, carefully inspect the various random accessories and parts in the package to avoid omissions. In order to save space and ensure safety of components, some components will be placed outside the inner packaging box, so be careful of their inspection.|
For special packaging, it is generally after opening the box, all packaging boxes, protective foam, plastic bags should be kept for a period of time. If there is a problem during the return period, you can return or exchange the original. After the return period (usually 10-30 days, according to the manufacturer’s Instruction of Terms of Service), these packaging boxes may be disposed of if there is no problem.
|Microscope Optical Data Sheet|
|P/N||Objective||Objective Working Distance||Eyepiece|
|Nexcope-NE620-Eyepiece-10 (10X Dia. 22mm)|
|Magnification||Field of View(mm)|
|1. Magnification=Objective Optical Magnification * Body Magnification * Eyepiece Optical Magnification|
|2. Field of View=Eyepiece Field of View /（Objective Optical Magnification*Body Magnification）|
|3. The Darker background items are Standard items, the white background items are optional items.|
|Video Microscope Optical Data Sheet|
|1. Magnification=Objective Optical Magnification * Body Magnification * Coupler Magnification|
|Desiccant Bag||1 Bag|
|Allen Key||M1.5 1pc|
|Product Instructions/Operation Manual||1pc|
|Packaging Type||Carton Packaging|
|Packaging Material||Corrugated Carton|
|Packaging Dimensions(1)||48x32x58cm (18.898x12.598x22.835″)|
|Inner Packing Material||Plastic Bag|
|Ancillary Packaging Materials||Expanded Polystyrene|
|Gross Weight||11.90kg (26.24lbs)|
|Minimum Packaging Quantity||1pc|
|Transportation Carton||Carton Packaging|
|Transportation Carton Material||Corrugated Carton|
|Transportation Carton Dimensions(1)||48x32x58cm (18.898x12.598x22.835″)|
|Total Gross Weight of Transportation(kilogram)||11.90|
|Total Gross Weight of Transportation(pound)||26.24|
|Quantity of One Transportation Carton||1pc|